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Distribution of Trop 3 and 4 Antigens as Defined by Monoclonal Antibodies Raised Against a Human Choriocarcinoma Cell Line
Author(s) -
SOUBIRAN PIERRE,
HSI BAELI,
LIPINSKI MARC,
YEH CHANGJING G.,
VAIGOT PIERRE,
MASSEYEFF RENE
Publication year - 1986
Publication title -
american journal of reproductive immunology and microbiology
Language(s) - English
Resource type - Journals
eISSN - 1600-0897
pISSN - 8755-8920
DOI - 10.1111/j.1600-0897.1986.tb00076.x
Subject(s) - monoclonal antibody , antigen , microbiology and biotechnology , biology , trophoblast , epithelium , cell culture , peripheral blood mononuclear cell , syncytiotrophoblast , antibody , immunology , placenta , in vitro , fetus , biochemistry , genetics , pregnancy
The reactivities of Trop 3 and 4 monoclonal antibodies (MAbs) were studied on human term and 7‐week extraembryonic membranes, adult tissues, and cell lines. Trop 3 MAb reacted with cells of chorionic villi, decidua, amniotic epithelium, and basal plate trophoblast. Trop 4 MAb reacted only with syncytiotrophoblast. On epithelium, Trop 3 MAb bound to stratified and glandular epithelium, but Trop 4 MAb reactivity was limited to basal keratinocytes. On peripheral blood mononuclear cells, Trop 3 MAb reacted with the majority of cells and Trop 4 MAb with the totality. Most cell lines were positive with both MAbs. However, one chemically induced MHC mutant was negative and another decreased its expression of Trop 3 antigen. Our results suggest Trop 3 MAb might recognize a monomorphic determinant of TLX antigens and Trop 4 is involved in cell proliferation or in cell‐to‐cell interaction.