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Immunoactive Products of Placenta: VIII. Translation Products of Messenger RNA Extracted From Murine Placenta or A6B9IC5 Teratocarcinoma Are Immunosuppressive In Vitro
Author(s) -
CHAOUAT G.,
KOLB J.P.,
RIVIERE M.,
LANKAR D.,
PROVOST M.A.
Publication year - 1985
Publication title -
american journal of reproductive immunology and microbiology
Language(s) - English
Resource type - Journals
eISSN - 1600-0897
pISSN - 8755-8920
DOI - 10.1111/j.1600-0897.1985.tb00274.x
Subject(s) - placenta , rna , xenopus , messenger rna , teratocarcinoma , in vitro , biology , microbiology and biotechnology , translation (biology) , andrology , biochemistry , gene , fetus , pregnancy , genetics , cellular differentiation , medicine
RNA was extracted from placentae of syn‐pregnant C3H mice (day 14 of gestation) and from A6B9IC5 teratocarcinoma. After isolation of poly‐(A)+ RNA on an oligo d(T) cellulose column, putative messenger RNA was injected into Xenopus laevi oocytes. Supernatants and homogenates from the oocytes were assayed for immunoregulatory activity in MLR, CML, and NK assays and found to be immunosuppressive. These data indicate that immunomodulators could be obtained from the placenta by molecular biology technology in sufficient quantities for eventual clinical applications.