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Immunological Specificity of Hexosaminidases From Human Seminal Plasma
Author(s) -
KAPUR DEEPAK K.,
GUPTA G.S.
Publication year - 1985
Publication title -
american journal of reproductive immunology and microbiology
Language(s) - English
Resource type - Journals
eISSN - 1600-0897
pISSN - 8755-8920
DOI - 10.1111/j.1600-0897.1985.tb00261.x
Subject(s) - isozyme , precipitin , antiserum , antigen , antibody , immunodiffusion , enzyme , ouchterlony double immunodiffusion , biochemistry , spleen , biology , epididymis , microbiology and biotechnology , epitope , chemistry , sperm , immunology , botany
ABSTRACT: Two isozymes of hexosaminidases (Hex) were purified from human seminal plasma and found to be homogeneous as revealed by immunoelectropho‐resis and immunodiffusion experiments. Anti‐Hex A antibodies were precipitated with isozyme B and vice versa. Though the precipitin arcs were equally stained for protein in these two antigen and antibody systems, enzyme activity was weakly stained in the reverse systems (ie, Anti‐Hex A with Hex B and Anti‐Hex B with Hex A). Thus, some common sequential antigenic determinants were indicated in two isozymes of Hex. On the other hand, loss of enzyme activity in the precipitate of reverse antigen‐antibody complexes as compared to direct system (Anti‐Hex A with Hex A and Anti‐Hex B with Hex B) revealed that the two isozymes are present in different sequential and conformational states near the active sites. Moreover, antibodies of both forms of Hex cross‐reacted with human sperm and reproductive and other vital organs such as kidney, liver, lung, pancreas, and spleen. Though monkey tissues also cross‐reacted with human seminal plasma Hex‐antisera, mouse, rat, and goat testis and epididymis extracts failed to show any cross‐reaction. It is concluded that human Hex(s) are tissue nonspecific but appear to be specific to the human and the subhuman primates.