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Incidence of Neutrophil Antigens on Human Cord Neutrophils
Author(s) -
Madyastha Prema R.,
Glassman Armand B.,
Levine David H.
Publication year - 1984
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
eISSN - 1600-0897
pISSN - 0271-7352
DOI - 10.1111/j.1600-0897.1984.tb00126.x
Subject(s) - immunology , cord blood , antigen , medicine , umbilical cord , antibody , neutropenia , neutrophile , neutrophil extracellular traps , granulocyte , inflammation , toxicity
ABSTRACT: Neutrophils isolated from cord blood of healthy newborns (33 blacks and 21 whites) were investigated by EDTA‐microagglutination for their expression of neutrophil specific antigens that have been associated with isoimmune neonatal or autoimmune neutropenia. Equal volumes of various neutrophil anti‐sera (2 μ) and cord neutrophils (3–5 × 10 6 /ml) were mixed in tissue typing microplates under oil and were incubated at room temperature for 6–8 hr, following which the degree of agglutination was noted. Our data revealed that all the currently recognized neutrophil antigens are readily demonstrable by antineutrophil antibodies in cord blood, (NA1, 52–54%; NA2, 81–85%; NB1, 95–96%; NC1, 90%; 9A, 29–30%) suggesting that neutrophil antigens are fully expressed at birth.