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Problems in the Measurement of Putative Serum Immune Complexes by the Method of Beaumont
Author(s) -
GOLDZIEHER JOSEPH W.,
GREENE NATHAN D.,
WILLIAMS MARY C.
Publication year - 1982
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
eISSN - 1600-0897
pISSN - 0271-7352
DOI - 10.1111/j.1600-0897.1982.tb00158.x
Subject(s) - immune system , antibody , ammonium sulfate , reproducibility , immunology , chemistry , chromatography , biology
A methodological investigation of the procedure used by Beaumont et al for measuring a “serum immune complex” precipitated by 25% ammonium sulfate and alleged to contain an ethynyl‐estradiol binding immunoglobulin has found major problems with reproducibility and with the correlation of total protein as measured by the Lowry method and the IgG content as determined by a specific nephelometric procedure.