Premium
Cell‐Mediated Immune Response of the Mammary Gland in Guinea Pigs. I. Effect of Antigen Injection Into the Vaccinated and Unvaccinated Glands *
Author(s) -
TARGOWSKI STANISLAW P.,
NECKE BRIAN J.
Publication year - 1982
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
eISSN - 1600-0897
pISSN - 0271-7352
DOI - 10.1111/j.1600-0897.1982.tb00081.x
Subject(s) - leukocytosis , immune system , antigen , immunology , peripheral blood mononuclear cell , biology , mammary gland , cell , lactation , in vitro , pregnancy , biochemistry , genetics , cancer , breast cancer
The leukocytic response of the mammary gland in the locally vaccinated guinea pigs to challenge with specific antigen during lactation was investigated. The response was measured by enumerating cells in milk collected at various times prior to and following antigenic challenge. A significant leukocytosis was observed in milk from vaccinated animals. The maximum cellular response by vaccinated animals was observed at 16 h to 30 h after challenge. The majority of leukocytes collected at that time did not form EA rosettes. Differential cell counts showed that the polymorphonuclear leukocytes were the major cell type until 30 h postchallenge while the mononuclear leukocytes predominated later. The delayed cellular response to challenge and the predominancy of leukocyte type at various times after challenge are discussed. It is proposed that the leukocyte response of the mammary glands in vaccinated animals was a cell‐mediated immune reaction.