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Important Role of the Carrier in the Induction of Antibody Response Without Freund's Complete Adjuvant Against a “Self” Peptide Luteinizing Hormone‐Releasing Hormone (LHRH) *
Author(s) -
SHASTRI N.,
MANHAR S. K.,
TALWAR G. P.
Publication year - 1981
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
eISSN - 1600-0897
pISSN - 0271-7352
DOI - 10.1111/j.1600-0897.1981.tb00051.x
Subject(s) - keyhole limpet hemocyanin , adjuvant , toxoid , hemocyanin , bovine serum albumin , luteinizing hormone , antibody , hormone , chemistry , peptide , medicine , endocrinology , immunology , biochemistry , immunization
Antibody response to the decapeptide luteinizing hormone‐releasing hormone (LHRH) was induced in mice without use of Freund's complete adjuvant. The conventionally employed conjugates of LHRH with bovine serum albumin were poor immunogens and required potent adjuvants. In contrast, LHRH linked covalently to tetanus toxoid or keyhole limpet hemocyanin‐generated antibodies with alum as adjuvant. Methods are described for preparation of LHRH conjugates with these carrier proteins with defined molar composition.