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Genetic Regulation of Antibody Response to Sheep Red Blood Cells: Linkage to H‐2 Complex
Author(s) -
ŘÍHOVÁ B.,
ŘÍHA I.
Publication year - 1981
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
eISSN - 1600-0897
pISSN - 0271-7352
DOI - 10.1111/j.1600-0897.1981.tb00030.x
Subject(s) - congenic , spleen , immunization , biology , antibody , microbiology and biotechnology , antigen , immunology , recombinant dna , gene , strain (injury) , genetics , anatomy
The analysis of anti‐sheep red blood cell (SRBC) antibody production in the congenic resistant (CR) mouse strains A/J and A.BY, B10 and B10.A showed that the level of IgG antibodies after immunization with SRBC is controlled by a gene(s) localized in the H‐2 complex. The use of the h2, h4, and i5 H‐2 recombinant haplotypes allowed us to map this gene into a region proximally defined by subregion I‐J and distally by region H‐2G. The IgG antibody level is simultaneously under the influence of non‐H‐2 genes, of which those of the A/J strain origin determine the high IgG level and those of the B10 strain origin determine the low IgG level. Weights of spleens of the A/J and B10 mice before and after primary and secondary immunization with SRBC were compared. Before immunization, spleens of the A/J mice were approximately 10% lighter than those of the B10 mice (0.08 g vs 0.09 g). After the first immunization the spleen weights equalized (θ = 0.12 g). Four days after the second immunization the weight of the spleens of A/J mice increased by 312% (relative to the nonimmune state) while that of the B10 mice increased by only 74%. These findings indicate that the cell antigen‐specific proliferation in spleens of A/J mice was considerably higher than that in spleens of B10 mice.