Premium
Human Soluble Antigens and Their Use in Sperm Antibody Testing *
Author(s) -
SHULMAN SIDNEY,
KEANE THOMAS
Publication year - 1981
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
eISSN - 1600-0897
pISSN - 0271-7352
DOI - 10.1111/j.1600-0897.1981.tb00019.x
Subject(s) - sperm , antigen , andrology , dithiothreitol , antibody , antiserum , agglutination (biology) , chemistry , trypsin , trypsin inhibitor , chromatography , latex fixation test , semen , immunology , biology , biochemistry , medicine , enzyme
Efforts were made to disrupt and solubilize human sperm cells and to evaluate the product for its content of infertility antibody‐related antigens. In the procedure that was developed, a well‐washed sperm sample is treated with 0.1 M dithiothreitol for 60 min, followed by trypsin at 0.1 mg/ml for 30 min, and then by soybean trypsin inhibitor. A mixture of DNAses I and II are added. After centrifuging, the resuspended pellet (RP) and the final supernatant (FS) show several degrees of cellular breakdown. Immunological evaluation was done with a strongly positive human serum containing sperm‐head antibody. From the inhibition of sperm agglutination, we could conclude that the desired soluble antigen was obtained in the FS fraction.