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Pex19p Contributes to Peroxisome Inheritance in the Association of Peroxisomes to Myo2p
Author(s) -
Otzen Marleen,
Rucktäschel Robert,
Thoms Sven,
Emmrich Kerstin,
Krikken Arjen M.,
Erdmann Ralf,
Klei Ida J.
Publication year - 2012
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1600-0854.2012.01364.x
Subject(s) - peroxisome , biology , inheritance (genetic algorithm) , microbiology and biotechnology , association (psychology) , genetics , gene , philosophy , epistemology
Abstract During budding of yeast cells peroxisomes are distributed over mother cell and bud, a process that involves the myosin motor protein Myo2p and the peroxisomal membrane protein Inp2p . Here, we show that Pex19p , a peroxin implicated in targeting and complex formation of peroxisomal membrane proteins, also plays a role in peroxisome partitioning. Binding studies revealed that Pex19p interacts with the cargo‐binding domain of Myo2p . We identified mutations in Myo2p that specifically reduced binding to Pex19p , but not to Inp2p . The interaction between Myo2p and Pex19p was also reduced by a mutation that blocked Pex19p farnesylation. Microscopy revealed that the Pex19p–Myo2p interaction is important for peroxisome inheritance, because mutations that affect this interaction hamper peroxisome inheritance in vivo . Together these data suggest that both Inp2p and Pex19p are required for proper association of peroxisomes to Myo2p .