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Protein Trafficking Rates Assessed by Quantum Dot Quenching with Bromocresol Green
Author(s) -
Valentine Cathleen D.,
Verkman A.S.,
Haggie Peter M.
Publication year - 2012
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1600-0854.2011.01287.x
Subject(s) - bromocresol green , quantum dot , quenching (fluorescence) , biology , cystic fibrosis transmembrane conductance regulator , fluorescence , biophysics , transmembrane protein , nanotechnology , biochemistry , receptor , chemistry , materials science , gene , chromatography , physics , quantum mechanics
Quantum dots are bright, photostable fluorophores used extensively to investigate biological processes. In this study, we report that bromocresol green (BCG) at low micromolar concentrations rapidly, efficiently and reversibly quenches the fluorescence of commercial quantum dots having a wide range of functionalities. The broad utility of BCG quenching of quantum dots in cell biology is showed in quantitative assays of trafficking of the β 2 ‐adrenergic receptor (β 2 AR) and the cystic fibrosis transmembrane conductance regulator (CFTR).