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Phagocytosis of IgG‐Coated Polystyrene Beads by Macrophages Induces and Requires High Membrane Order
Author(s) -
Magenau Astrid,
Benzing Carola,
Proschogo Nicholas,
Don Anthony S.,
Hejazi Leila,
Karunakaran Denuja,
Jessup Wendy,
Gaus Katharina
Publication year - 2011
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1600-0854.2011.01272.x
Subject(s) - phagosome , phagocytosis , membrane , sphingomyelin , microbiology and biotechnology , biology , biophysics , ceramide , cell membrane , actin , actin cytoskeleton , pinocytosis , endocytosis , cytoskeleton , biochemistry , cell , apoptosis
The biochemical composition and biophysical properties of cell membranes are hypothesized to affect cellular processes such as phagocytosis. Here, we examined the plasma membranes of murine macrophage cell lines during the early stages of uptake of immunoglobulin G (IgG)‐coated polystyrene particles. We found that the plasma membrane undergoes rapid actin‐independent condensation to form highly ordered phagosomal membranes, the biophysical hallmark of lipid rafts. Surprisingly, these membranes are depleted of cholesterol and enriched in sphingomyelin and ceramide. Inhibition of sphingomyelinase activity impairs membrane condensation, F‐actin accumulation at phagocytic cups and particle uptake. Switching phagosomal membranes to a cholesterol‐rich environment had no effect on membrane condensation and the rate of phagocytosis. In contrast, preventing membrane condensation with the oxysterol 7‐ketocholesterol, even in the presence of ceramide, blocked F‐actin dissociation from nascent phagosomes and particle uptake. In conclusion, our results suggest that ordered membranes function to co‐ordinate F‐actin remodelling and that the biophysical properties of phagosomal membranes are essential for phagocytosis.