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The Role of VAMP7/TI‐VAMP in Cell Polarity and Lysosomal Exocytosis in vivo
Author(s) -
Sato Mahito,
Yoshimura Shinichiro,
Hirai Rika,
Goto Ayako,
Kunii Masataka,
Atik Nur,
Sato Takashi,
Sato Ken,
Harada Reiko,
Shimada Junko,
Hatabu Toshimitsu,
Yorifuji Hiroshi,
Harada Akihiro
Publication year - 2011
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1600-0854.2011.01247.x
Subject(s) - exocytosis , microbiology and biotechnology , biology , rab , munc 18 , cell polarity , epithelial polarity , knockout mouse , axoplasmic transport , transport protein , endosome , synaptic vesicle , vesicle , cell , intracellular , gtpase , secretion , biochemistry , receptor , membrane
VAMP7 or tetanus neurotoxin‐insensitive vesicle‐ associated membrane protein (TI‐VAMP) has been proposed to regulate apical transport in polarized epithelial cells, axonal transport in neurons and lysosomal exocytosis. To investigate the function of VAMP7 in vivo , we generated VAMP7 knockout mice. Here, we show that VAMP7 knockout mice are indistinguishable from control mice and display a similar localization of apical proteins in the kidney and small intestine and a similar localization of axonal proteins in the nervous system. Neurite outgrowth of cultured mutant hippocampal neurons was reduced in mutant neurons. However, lysosomal exocytosis was not affected in mutant fibroblasts. Our results show that VAMP7 is required in neurons to extend axons to the full extent. However, VAMP7 does not seem to be required for epithelial cell polarity and lysosomal exocytosis.