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Generic Sorting of Raft Lipids into Secretory Vesicles in Yeast
Author(s) -
Surma Michal A.,
Klose Christian,
Klemm Robin W.,
Ejsing Christer S.,
Simons Kai
Publication year - 2011
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1600-0854.2011.01221.x
Subject(s) - vesicle , sphingolipid , biology , raft , secretory pathway , golgi apparatus , microbiology and biotechnology , lipid raft , secretory vesicle , lipid microdomain , endosome , secretion , biochemistry , membrane , chemistry , endoplasmic reticulum , intracellular , organic chemistry , copolymer , polymer
Previous work has showed that ergosterol and sphingolipids become sorted to secretory vesicles immunoisolated using a chimeric, artificial raft membrane protein as bait. In this study, we have extended this analysis to three populations of secretory vesicles isolated using natural yeast plasma membrane (PM) proteins: Pma1p, Mid2p and Gap1*p as baits. We compared the lipidomes of the immunoisolated vesicles with each other and with the lipidomes of the donor compartment, the trans ‐Golgi network, and the acceptor compartment, the PM, using a quantitative mass spectrometry approach that provided a complete lipid overview of the yeast late secretory pathway. We could show that vesicles captured with different baits carry the same cargo and have almost identical lipid compositions; being highly enriched in ergosterol and sphingolipids. This finding indicates that lipid raft sorting is a generic feature of vesicles carrying PM cargo and suggests a common lipid‐based mechanism for their formation.