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Dynamics of Native β‐actin mRNA Transport in the Cytoplasm
Author(s) -
Lifland Aaron W.,
Zurla Chiara,
Yu Joana,
Santangelo Philip J.
Publication year - 2011
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1600-0854.2011.01209.x
Subject(s) - biology , cytoplasm , microbiology and biotechnology , translation (biology) , microtubule , messenger rna , fluorescence recovery after photobleaching , actin , rna , biophysics , biochemistry , gene , membrane
Transport of messenger RNAs (mRNAs) in the cytoplasm is essential for localization to translation sites and for post‐transcriptional regulation. Utilizing single‐RNA sensitive probes and real‐time fluorescence microscopy, we accurately quantified the dynamics of native, non‐engineered, β‐actin mRNAs within the cytoplasm of epithelial cells and fibroblasts for the first time. Using single‐particle tracking and temporal analysis, we determined that native β‐actin mRNAs, under physiologic conditions, exhibit bursts of intermittent, processive motion on microtubules, interspersed between time periods of diffusive motion, characterized by non‐thermal enhanced diffusivity. When transport processes were perturbed via ATP depletion, temperature reduction, dynamitin overexpression and chemical inhibitors, processive motion was diminished or eliminated and diffusivity was reduced. These data support a model whereby processive, motor‐driven motion is responsible for long‐distance mRNA transport.