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Trafficking Properties of the D5 Dopamine Receptor
Author(s) -
Thompson Dawn,
Whistler Jennifer L.
Publication year - 2011
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1600-0854.2011.01179.x
Subject(s) - endocytosis , microbiology and biotechnology , biology , receptor , endocytic cycle , enzyme linked receptor , dopamine receptor , agonist , d1 like receptor , signal transduction , g protein coupled receptor , 5 ht5a receptor , endogenous agonist , d2 like receptor , clathrin , dopamine receptor d1 , biochemistry
Dopamine receptors are important for diverse biological functions and are important pharmacological targets in human medicine. Signal transduction from the dopamine receptors is controlled at many levels, including by the process of receptor trafficking. Little is known regarding the endocytic and postendocytic trafficking properties of the D5 dopamine receptor. Here, we show that endocytosis of the D5 receptor can be achieved both homologously, through direct receptor activation by agonist, and also heterologously, due to independent activation of protein kinase C (PKC). In contrast, the D1 receptor is endocytosed only in response to agonist but not PKC activation. We have identified the residue in the third intracellular loop of the D5 receptor that is both necessary for PKC‐mediated endocytosis of the D5 receptor and sufficient to induce PKC‐mediated endocytosis when introduced to the D1 receptor. In addition, we show that endocytosis of D5 through both pathways is dependent on clathrin and dynamin but that only agonist‐induced endocytosis engages β‐arrestin 2. Together, these data show that the D5 receptor shows a trafficking profile distinct from that of any of the other dopamine receptors.