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Live Cell Multicolor Imaging of Lipid Droplets with a New Dye, LD540
Author(s) -
Spandl Johanna,
White Daniel J.,
Peychl Jan,
Thiele Christoph
Publication year - 2009
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1600-0854.2009.00980.x
Subject(s) - lipid droplet , fluorophore , live cell imaging , bodipy , biophysics , fluorescence , biology , motility , microbiology and biotechnology , microtubule , cell , optics , biochemistry , physics
A lipophilic dye based on the Bodipy fluorophore, LD540, was developed for microscopic imaging of lipid droplets. In contrast to previous lipid droplet dyes, it can spectrally be resolved from both green and red fluorophores allowing multicolor imaging in both fixed and living cells. Its improved specificity, brightness and photostability support live cell imaging, which was used to demonstrate by two‐color imaging lipid droplet motility along microtubules.