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btn1 Affects Endocytosis, Polarization of Sterol‐Rich Membrane Domains and Polarized Growth in Schizosaccharomyces pombe
Author(s) -
Codlin Sandra,
Haines Rebecca L.,
Mole Sara E.
Publication year - 2008
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1600-0854.2008.00735.x
Subject(s) - schizosaccharomyces pombe , biology , schizosaccharomyces , endocytosis , microbiology and biotechnology , cytokinesis , endomembrane system , vacuole , organelle , actin , cell polarity , cell membrane , cell , saccharomyces cerevisiae , cell division , endoplasmic reticulum , golgi apparatus , biochemistry , yeast , cytoplasm
btn1 , the Schizosaccharomyces pombe orthologue of the human Batten disease gene CLN3 , exerts multiple cellular effects. As well as a role in vacuole pH homoeostasis, we now show that Btn1p is essential for growth at high temperatures. Its absence results in progressive defects at 37°C that culminate in total depolarized growth and cell lysis. These defects are preceded by a progressive failure to correctly polarize sterol‐rich domains after cytokinesis and are accompanied by loss of Myo1p localization. Furthermore, we found that in Sz. pombe , sterol spreading is linked to defective formation/polarization of F‐actin patches and disruption of endocytosis and that these processes are aberrant in btn1 Δ cells. Consistent with a role for Btn1p in polarized growth, Btn1p has an altered location at 37°C and is retained in actin‐dependent endomembrane structures near the cell poles or septum.