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Lack of a Vacuolar Sorting Receptor Leads to Non‐Specific Missorting of Soluble Vacuolar Proteins in Arabidopsis Seeds
Author(s) -
Craddock Christian P.,
Hunter Paul R.,
Szakacs Erika,
Hinz Giselbert,
Robinson David G.,
Frigerio Lorenzo
Publication year - 2008
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1600-0854.2007.00693.x
Subject(s) - biology , arabidopsis , vacuole , protein targeting , microbiology and biotechnology , vacuolar protein sorting , secretion , protein sorting signals , mutant , sorting , arabidopsis thaliana , secretory protein , receptor , gene isoform , cytoplasm , biochemistry , signal peptide , gene , peptide sequence , membrane protein , membrane , computer science , programming language
The plant vacuolar sorting receptor (VSR) binds proteins carrying vacuolar sorting signals (VSS) of the ‘sequence‐specific’ type (ssVSS) but not the C‐terminal, hydrophobic sorting signals (ctVSS). Seeds of Arabidopsis mutants lacking the major VSR isoform, AtVSR1, secrete a proportion of the proteins destined to storage vacuoles. The sorting signals for these proteins are not well defined, but they do not seem to be of the ssVSS type. Here, we tested whether absence of VSR1 in seeds leads to secretion of reporter proteins carrying ssVSS but not ctVSS. Our results show that reporters carrying either ssVSS or ctVSS are equally secreted in the absence of VSR1. We discuss our findings in relation to the current model for vacuolar sorting.