Premium
Overexpression of a Plant Reticulon Remodels the Lumen of the Cortical Endoplasmic Reticulum but Does not Perturb Protein Transport
Author(s) -
Tolley Nicholas,
Sparkes Imogen A.,
Hunter Paul R.,
Craddock Christian P.,
Nuttall James,
Roberts Lynne M.,
Hawes Chris,
Pedrazzini Emanuela,
Frigerio Lorenzo
Publication year - 2008
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1600-0854.2007.00670.x
Subject(s) - endoplasmic reticulum , fluorescence recovery after photobleaching , biology , golgi apparatus , microbiology and biotechnology , yellow fluorescent protein , arabidopsis thaliana , secretion , transport protein , membrane protein , subcellular localization , lumen (anatomy) , er retention , biochemistry , cytoplasm , membrane , gene , mutant
We have cloned a member of the reticulon (RTN) family of Arabidopsis thaliana (RTNLB13). When fused to yellow fluorescent protein (YFP) and expressed in tobacco leaf epidermal cells, RTNLB13 is localized in the endoplasmic reticulum (ER). Coexpression of a soluble ER luminal marker reveals that YFP‐tagged, myc‐tagged or untagged RTNLB13 induces severe morphological changes to the lumen of the ER. We show, using fluorescence recovery after photobleaching (FRAP) analysis, that RTNLB13 overexpression greatly reduces diffusion of soluble proteins within the ER lumen, possibly by introducing constrictions into the membrane. In spite of this severe phenotype, Golgi shape, number and dynamics appear unperturbed and secretion of a reporter protein remains unaffected.