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A Bioinformatics Approach to Identifying Tail‐Anchored Proteins in the Human Genome
Author(s) -
Kalbfleisch Ted,
Cambon Alex,
Wattenberg Binks W.
Publication year - 2007
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1600-0854.2007.00661.x
Subject(s) - biology , ferm domain , transmembrane protein , transmembrane domain , membrane protein , microbiology and biotechnology , protein targeting , fusion protein , protein domain , genome , amino acid , computational biology , gene , integral membrane protein , genetics , membrane , recombinant dna , receptor
Intracellular proteins with a carboxy‐terminal transmembrane domain and the amino‐terminus oriented toward the cytosol are known as ‘tail‐anchored’ proteins. Tail‐anchored proteins have been of considerable interest because several important classes of proteins, including the vesicle‐targeting/fusion proteins known as SNAREs and the apoptosis‐related proteins of the Bcl‐2 family, among others, utilize this unique membrane‐anchoring motif. Here, we use a bioinformatic technique to develop a comprehensive list of potentially tail‐anchored proteins in the human genome. Our final list contains 411 entries derived from 325 unique genes. We also analyzed both known and predicted tail‐anchored proteins with respect to the amino acid composition of the transmembrane segments. This analysis revealed a distinctive composition of the membrane anchor in SNARE proteins.