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Differential Requirements for ts‐O45‐G and Procollagen Biosynthetic Transport
Author(s) -
Starkuviene Vytaute,
Pepperkok Rainer
Publication year - 2007
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1600-0854.2007.00582.x
Subject(s) - biology , differential (mechanical device) , microbiology and biotechnology , computational biology , engineering , aerospace engineering
Emerging experimental evidence favours the existence of cargo sorting occurring upon the endoplasmic reticulum (ER) exit. Recent studies revealed that, in contrast to the conventional secretory marker ts‐O45‐G, procollagen (PC I) exits the ER at sites not coated with coat protein II and is transported to the Golgi complex in carriers devoid of coat protein I. Here, we investigated whether PC I trafficking requires a different molecular machinery in comparison with the ts‐O45‐G. By combining colocalization of the cargoes with endogenous markers, downregulation of transport machinery by RNA interference and knock‐ins by complementary DNA over‐expression, we provide strong evidence that PC I and ts‐O45‐G have common but also different molecular requirements during pre‐ and post‐Golgi trafficking events.