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A Novel Kinesin‐Like Protein, KIF1Bβ3 Is Involved in the Movement of Lysosomes to the Cell Periphery in Non‐Neuronal Cells
Author(s) -
Matsushita Masafumi,
Tanaka Shingo,
Nakamura Norihiro,
Inoue Hiroki,
Kanazawa Hiroshi
Publication year - 2004
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1600-0854.2003.00165.x
Subject(s) - kinesin , biology , gene isoform , microbiology and biotechnology , mutation , exon , alternative splicing , gene , genetics , microtubule
The kinesin superfamily protein, KIF1Bβ, a splice variant of KIF1B, is involved in the transport of synaptic vesicles in neuronal cells, and is also expressed in various non‐neuronal tissues. To elucidate the functions of KIF1Bβ in non‐neuronal cells, we analyzed the intracellular localization of KIF1Bβ and characterized its isoform expression profile. In COS‐7 cells, KIF1B colocalized with lysosomal markers and expression of a mutant form of KIF1Bβ, lacking the motor domain, impaired the intracellular distribution of lysosomes. A novel isoform of the kinesin‐like protein, KIF1Bβ3, was identified in rat and simian kidney. It lacks the 5th exon of the KIF1Bβ‐specific tail region. Overexpression of KIF1Bβ3 induced the translocation of lysosomes to the cell periphery. However, overexpression of KIF1Bβ3‐Q98L, which harbors a pathogenic mutation associated with a familial neuropathy, Charcot‐Marie‐Tooth disease type 2 A, resulted in the abnormal perinuclear clustering of lysosomes. These results indicate that KIF1Bβ3 is involved in the translocation of lysosomes from perinuclear regions to the cell periphery.