Open AccessSulfur mustard cutaneous injury characterization based on SKH ‐1 mouse model: relevance of non‐invasive methods in terms of wound healing process analyses
Author(s) -
CléryBarraud Cécile,
Nguon Nina,
Vallet Virginie,
Sentenac Catherine,
Four Elise,
Arlaud Carine,
Coulon David,
Boudry Isabelle
Publication year - 2013
Publication title -
skin research and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.521
H-Index - 69
eISSN - 1600-0846
pISSN - 0909-752X
DOI - 10.1111/j.1600-0846.2012.00621.x
Subject(s) - transepidermal water loss , weanling , wound healing , sulfur mustard , animal model , toxicity , dermatology , pathology , medicine , surgery , veterinary medicine , stratum corneum
Background To date, sulphur mustard ( SM ) cutaneous toxicity has been commonly assessed on account of several animal models such as pigs and weanling pigs. Few experiments however, have been carried out on mice so far. In this study, we aimed at quantifying spontaneous wound healing processes after SM exposure on a SKH ‐1 mouse model through non‐invasive methods over an extended period of time. Methods Animals were exposed to 10 μL net SM in a vapor cup system. Measurements of barrier function (Transepidermal water loss), elasticity, skin color exposed to SM vapors were determined by evaporimetry, cutometer and image analysis on 23 animals up to 28 days. Results were subsequently correlated with histological and biochemical analyses. Results The TEWL parameter stands as a top‐ranking criterion to keep track of skin barrier restoration after SM cutaneous intoxication in our SKH ‐1 mouse model. The R2 and R6 elasticity parameters or L° for the skin color exhibited their ability to be restored after 28 days of SM exposure. Conclusion Our findings suggest that bio‐engineering methods are eligible to evaluate new treatments on SM ‐induced skin SKH ‐1 mouse lesions, thus making an allowance for less invasive methods such as histological, genomic or proteomic approaches.