Open AccessEvaluation of allergic vesicular reaction to patch test using in vivo confocal microscopy
Author(s) -
Ardigò Marco,
Longo Caterina,
Cristaudo Antonio,
Berardesca Enzo,
Pellacani Giovanni
Publication year - 2012
Publication title -
skin research and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.521
H-Index - 69
eISSN - 1600-0846
pISSN - 0909-752X
DOI - 10.1111/j.1600-0846.2011.00531.x
Subject(s) - confocal microscopy , confocal , microscopy , in vivo , pathology , medicine , chemistry , biology , optics , microbiology and biotechnology , physics
Background: Confocal microscopy has been successfully applied both in oncologic and inflammatory diseases. In particular, it has been proved as a useful tool for the in vivo detection of microscopical changes occurring in allergic reactions.Aims of the study: To evaluate microscopic changes occurring in positive patch test reactions.Methods: Eight patients with history of allergic dermatitis and positive patch test reaction were analysed by means of confocal microscopy.Results: Confocal microscopy showed the presence of spongiotic vesicle preferentially localized around the adnexal ducts that appeared to be in the middle of the spongiotic phenomena.Conclusion: Confocal microscopy offered for the first time new insight into vesicle formation and development, showing that adnexal ducts can play a role in allergic reaction.