Open AccessQuantification of stratum corneum removal by adhesive tape stripping by total protein assay in 96‐well microplates
Author(s) -
Dreher F.,
Modjtahedi B. S.,
Modjtahedi S. P.,
Maibach H. I.
Publication year - 2005
Publication title -
skin research and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.521
H-Index - 69
eISSN - 1600-0846
pISSN - 0909-752X
DOI - 10.1111/j.1600-0846.2005.00103.x
Subject(s) - stratum corneum , chromatography , adhesive , stripping (fiber) , chemistry , materials science , composite material , medicine , layer (electronics) , pathology
Background/aims: Examination of stratum corneum (SC) content with tape stripping and a colorimetric method is increasingly used. We examined the possible use of microplates in tandem with a colorimetric method to examine SC removed with tape stripping. As a corollary to this examination, the homogeneity of tape strips was examined. Method: The commonly used Lowry assay was adapted for 96‐well plates. Tapes were divided into four regions and sample disks of 5 mm diameter were taken from each and analyzed for SC mass using the adapted Lowry assay. Results: Homogeneity of SC removal over different areas across a tape strip is limited. Conclusion: Quantification of SC by means of a 96‐well microplate‐based colorimetric method is feasible and shortens the time of analysis. However, when using D‐Squame ® tape disks, SC removal on a limited area of the tape is not predictive for SC removal on the entire tape as removal is inhomogenous. Therefore, SC protein extraction should be performed on a large enough area, eventually on the entire tape when quantifying SC mass removed by tape stripping.