Open AccessComparative study of five instruments measuring stratum corneum hydration (Corneometer CM 820 and CM 825, Skicon 200, Nova DPM 9003, DermaLab). Part I. In vitro
Author(s) -
Fluhr Joachim W.,
Gloor Max,
Lazzerini Sabrina,
Kleesz Peter,
Grieshaber Romano,
Berardesca Enzo
Publication year - 1999
Publication title -
skin research and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.521
H-Index - 69
eISSN - 1600-0846
pISSN - 0909-752X
DOI - 10.1111/j.1600-0846.1999.tb00126.x
Subject(s) - desorption , stratum corneum , dielectric , analytical chemistry (journal) , nova (rocket) , materials science , sorption , chemistry , mineralogy , chromatography , adsorption , optoelectronics , medicine , pathology , aeronautics , engineering
Background/aims: The hydration state of stratum corneum can be measured with different instruments. In the present study, five instruments were compared under different experimental in vitro conditions: Corneometer CM 820 and CM 825, Skicon 200, Nova DPM and DermaLab. Methods: A sorption‐desorption test, measurements on different filters and with liquids with different dielectric constants, measurement‐depth analysis and measurements on different agar plates were performed. Results: Good correlation between the values of the five instruments and μl (sorption test: varying from r = 0.8249 to r = 0.9635) and time (desorption test: correlation varying from r = 0.7615 to r =0.9909) could be demonstrated. A high Spearman correlation coefficient ( r ) could be proven for dielectric constant (dc)/CM 820 ( r =0.9333), dc/CM 825 ( r =0.9833) and dc/Nova DPM ( r =0.9048). A lower correlation or no correlation was found for dc/DermaLab ( r =0.8469; P =0.023) and dc/Skicon 200 ( r =0.9000; P =0.083). Conclusions: For each of the five instruments, the electrical field of measurement can be assumed to cover the following depths: CM 820, between 30 and 45 μm; CM 825, around or less than 15 μm; Skicon 200, less than 15 μm; Nova DPM, between 45 and 60 μm; DermaLab, around or less than 15 nm.