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Training alters the skeletal muscle antioxidative capacity in non‐insulin‐dependent type 2 diabetic men
Author(s) -
Brinkmann C.,
Chung N.,
Schmidt U.,
Kreutz T.,
Lenzen E.,
Schiffer T.,
Geisler S.,
Graf C.,
MontielGarcia G.,
Renner R.,
Bloch W.,
Brixius K.
Publication year - 2012
Publication title -
scandinavian journal of medicine and science in sports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.575
H-Index - 115
eISSN - 1600-0838
pISSN - 0905-7188
DOI - 10.1111/j.1600-0838.2010.01273.x
Subject(s) - endocrinology , medicine , skeletal muscle , sod2 , gpx1 , glutathione peroxidase , peroxiredoxin , overweight , insulin resistance , type 2 diabetes , type 2 diabetes mellitus , insulin , oxidative stress , diabetes mellitus , superoxide dismutase , body mass index , biology , peroxidase , biochemistry , enzyme
The present study analyzes the oxidative stress situation in the skeletal muscle of overweight/obese men suffering from non‐insulin‐dependent type 2 diabetes mellitus [T2DM, n =16, years=61±7, body mass index (BMI)=31±4 kg/m 2 ] and BMI‐matched non‐diabetic male control subjects (CON, n =7, years=53±6, BMI=30±4 kg/m 2 ). Furthermore, it investigates whether physical training can alter the skeletal muscle antioxidative capacity of T2DM patients at rest. Molecule content analyses (immunohistochemical stainings) of 8‐iso‐prostaglandin‐F2α (8‐Iso‐PGF), superoxide dismutase‐2 (SOD2), glutathione peroxidase‐1 (GPX1), peroxiredoxin isoforms (PRDX 1–6) and heat‐shock‐protein‐70 (HSP70) were performed in biopsies taken from the vastus lateralis muscle. Under basal conditions, 8‐Iso‐PGF was significantly decreased in T2DM patients (−35.7%), whereas PRDX2 and PRDX6 were significantly increased relative to CON (+82.6%; +82.3%). Differences were neither observed in SOD2 nor in GPX1 or PRDX1, 3, 4, 5 density. Regular physical activity (moderate endurance or resistance training twice a week for 3 months) did not alter PRDX1, 2, 3, 4, 6 in the skeletal muscle of T2DM patients, but significantly increased SOD2 (+65.9%), GPX1 (+62.4%), PRDX5 (+37.5%), and HSP70 (+48.5%). Overweight/obese men with non‐insulin‐dependent T2DM exhibit up‐regulated cytosolic peroxiredoxin contents relative to BMI‐matched controls. Regular training further up‐regulates cytosolic and mitochondrial antioxidative enzymes in T2DM patients and improves their cellular protection systems. This may contribute to a retardation of the disease's progression.