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Dependence of hemoglobin mass estimation with the optimized CO‐rebreathing method on different spectrophotometers
Author(s) -
Ulrich G,
Strunz J,
Frese F,
Bärtsch P,
FriedmannBette B
Publication year - 2012
Publication title -
scandinavian journal of medicine and science in sports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.575
H-Index - 115
eISSN - 1600-0838
pISSN - 0905-7188
DOI - 10.1111/j.1600-0838.2010.01247.x
Subject(s) - carboxyhemoglobin , chemistry , analytical chemistry (journal) , hemoglobin , chromatography , carbon monoxide , biochemistry , catalysis
The assessment of total hemoglobin mass (tHb‐mass) with the optimized carbon monoxide‐rebreathing procedure (oCOR) is discussed as a promising method to detect blood doping. The method requires repeated measurements of the carboxyhemoglobin fraction (%HbCO) using spectrophotometers (CO oximeters). In order to determine whether %HbCO measurements with different spectrophotometers yield similar tHb‐masses, the results of 57 tHb‐mass calculations from simultaneous %HbCO measurements with two different spectrophotometers (RapidLab, OSM3) were analyzed. For the comparison of longitudinal tHb‐mass alterations (ΔtHb‐mass), 3 tHb‐mass measurements were obtained at 6‐month intervals (33–37 subjects). Because of significant differences in %HbCO measurements, the limits of agreement for tHb‐mass OSM3 and tHb‐mass RapidLab were 11.2% (95% reference range −6.8 to +15.6%) and the correlation of ΔtHb‐masses as determined with the two spectrophotometers over two time intervals was weak ( r : 0.28–0.66). In only about 70% of all ΔtHb‐mass estimations did ΔtHb‐mass OSM3 and ΔtHb‐mass RapidLab show the same direction of change. Apparently, the analytical variation in tHb‐mass determination with oCOR increases considerably with the use of different spectrophotometers. Therefore, agreement on the use of one spectrophotometer that accurately measures low %HbCO values is needed if oCOR should be used in an anti‐doping setting.