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Plasma antioxidant responses and oxidative stress following a soccer game in elite female players
Author(s) -
Andersson H.,
Karlsen A.,
Blomhoff R.,
Raastad T.,
Kadi F.
Publication year - 2010
Publication title -
scandinavian journal of medicine and science in sports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.575
H-Index - 115
eISSN - 1600-0838
pISSN - 0905-7188
DOI - 10.1111/j.1600-0838.2009.00987.x
Subject(s) - ascorbic acid , uric acid , glutathione , lipid peroxidation , oxidative stress , chemistry , antioxidant , medicine , endocrinology , carotenoid , biochemistry , food science , enzyme
We aimed to investigate markers of oxidative stress and levels of endogenous and dietary antioxidants in 16 elite female soccer players in response to a 90‐min game (average intensity 82±3% HR peak ). Blood samples were taken before, immediately and 21 h after the game. Plasma‐oxidized glutathione, the ratio of reduced to oxidized glutathione (GSH:GSSG) and lipid peroxidation measured by d‐ROMs were used as markers of oxidative stress. Plasma endogenous [uric acid, total glutathione (TGSH)] and dietary antioxidants (α‐tocopherol, ascorbic acid, total carotenoids and polyphenols) were analyzed using liquid chromatography and the Folin–Ciocalteu method. Exercise induced an acute increase ( P <0.05) in GSSG, uric acid, TGSH, α‐tocopherol, and ascorbic acid. In parallel, the GSH:GSSG ratio and polyphenols decreased ( P <0.05). GSSG, GSH:GSSG ratio, uric acid, TGSH, and ascorbic acid returned to baseline at 21 h, while polyphenols and α‐tocopherol remained altered. Total carotenoids increased above baseline only at 21 h ( P <0.05). Lipid peroxidation, measured by d‐ROMs, remained unchanged throughout the study. Thus, intermittent exercise in well‐trained female athletes induces a transient increase in GSSG and a decrease in the GSH:GSSG ratio, which is effectively balanced by the recruitment of both endogenous and dietary antioxidants, resulting in the absence of lipid peroxidation measured by d‐ROMs.