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Melatonin prolongs graft survival of pancreas allotransplants in pigs
Author(s) -
GarcíaGil Francisco A.,
Albendea Carlos D.,
Escartín Jorge,
Lampreave Fermin,
FuentesBroto Lorena,
RosellóCatafau Joan,
LópezPingarrón Laura,
Reiter Russel J.,
AlvarezAlegret Ramiro,
García Joaquín J.
Publication year - 2011
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/j.1600-079x.2011.00908.x
Subject(s) - melatonin , transplantation , malondialdehyde , oxidative stress , medicine , immunosuppression , cold storage , ascorbic acid , reperfusion injury , endocrinology , ischemia , surgery , andrology , biology , food science , horticulture
Oxidative stress is involved in ischemia‐reperfusion injury and allograft rejection after transplantation. We studied two well‐known antioxidants, melatonin and ascorbic acid (AA), in relation to the survival of a pancreas transplantation model without immunosuppression. Forty‐eight Landrace pigs were divided into three groups (n = 16 each; eight donors and eight recipients) that received melatonin, AA, or no antioxidant therapy (controls). Melatonin and AA were administered (10 mg/kg body weight) intravenously to donors and recipients during surgery and on postoperative days 1–7. The molecules were also added (5 m m ) to a University of Wisconsin preservation solution during organ cold storage. Melatonin significantly delayed acute rejection and prolonged allograft survival (25.1 ± 7.7 days) compared with the controls (8.1 ± 0.8 days, P = 0.013) and the AA group (9.4 ± 1.6 days, P = 0.049). Melatonin reduced indicators of oxidative stress, malondialdehyde, and 4‐hydroxyalkenals, in pancreatic samples collected during procurement, cold ischemia, and reperfusion. Melatonin also reduced serum pig‐major acute‐phase protein/inter‐α‐trypsin inhibitor heavy chain 4 (pMAP/ITIH 4 ) in the early post‐transplantation period. AA only partially reduced oxidative damage 30 min postreperfusion and failed to prevent pMAP/ITIH 4 elevations. These findings suggested that melatonin may be a useful therapeutic tool for organ transplantation.