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Melatonin protects against hydrogen peroxide‐induced cell death signaling in SH‐SY5Y cultured cells: involvement of nuclear factor kappa B, Bax and Bcl‐2
Author(s) -
Chetsawang Banthit,
Putthaprasart Chorkaew,
PhansuwanPujito Pansiri,
Govitrapong Piyarat
Publication year - 2006
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/j.1600-079x.2006.00335.x
Subject(s) - melatonin , sh sy5y , viability assay , oxidative stress , neuroprotection , programmed cell death , apoptosis , microbiology and biotechnology , biology , chemistry , cell culture , biochemistry , endocrinology , pharmacology , neuroblastoma , genetics
Oxidative stress is defined as a disturbance in the prooxidant–antioxidant balance, leading to potential cell damage. Reactive oxygen species such as superoxide radicals, hydroxyl radicals and hydrogen peroxide may act also as secondary intermediaries in intracellular signaling leading to cell death. The neuroprotective effect of melatonin has been observed both in vivo and in vitro. The objective of this research, therefore, was to better understand the cellular mechanisms of neuronal cell degeneration induced via oxidative stress and the protective roles of melatonin on this cell death. In the present study, the effects of melatonin on H 2 O 2 ‐induced neuronal cell degeneration in human dopaminergic neuroblastoma SH‐SY5Y cultured cells were investigated. The results showed that H 2 O 2 significantly decreased cell viability and melatonin reversed the toxic effects of H 2 O 2 . An inhibition of caspase enzyme activity by Ac‐DEVD‐CHO, a caspase‐3 inhibitor, significantly increased cell viability in H 2 O 2 ‐treated cells. The phosphorylation of transcription factors, nuclear factor kappa B (NF‐ κ B) was increased in H 2 O 2 ‐treated cells and this effect was abolished by melatonin. Translocation of phosphorylated NF‐ κ B to perinuclear and nuclear sites, estimated using immunofluorescence, occurred to a greater extent in H 2 O 2 ‐treated cells than in untreated control cells and again this effect was abolished by melatonin. In addition, induction of Bcl‐2 and Bax proteins was demonstrated in SH‐SY5Y cultured cells treated with H 2 O 2 , whereas the induction of Bax but not Bcl‐2 was diminished by melatonin. In light of these finding, it is possible that the antioxidative stress effect of melatonin associated with inhibition of Bax expression, may offer a means of treating neuronal degeneration and disease.