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Microarray analysis of transcription factor gene expression in melatonin‐treated human peripheral blood mononuclear cells
Author(s) -
Ha Eunyoung,
Han Earl,
Park Hae Jeong,
Kim HakJae,
Hong Mee Suk,
Hong SeungJae,
Yoon Kyung Sik,
Kang Insug,
Cho Yong Hoo,
Chung JooHo,
Yim SungVin,
Baik Hyung Hwan
Publication year - 2006
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/j.1600-079x.2006.00317.x
Subject(s) - melatonin , biology , transcription factor , microarray analysis techniques , peripheral blood mononuclear cell , microarray , gene expression , melatonin receptor , downregulation and upregulation , microbiology and biotechnology , immune system , gene , immunology , endocrinology , in vitro , genetics
The existence of specific melatonin‐binding sites in lymphoid cells led to the discovery of signal transduction pathway for melatonin in human lymphocytes and immunomodulatory role of melatonin in immune cells. In recent years, transcriptional regulation of melatonin on various transcription factors has been demonstrated. Therefore, this study was designed to assess by cDNA microarray analysis the regulatory effects of melatonin on transcription factors in human peripheral blood mononuclear cells (PBMCs). Forty‐six genes were upregulated and 23 were downregulated more than twofold in melatonin‐treated PBMCs. Of the more than twofold upregulated transcription factor genes, homeo box A4 ( HOXA4 ), forkhead box O1A ( FOXO1A ), transcription elongation factor B (SIII), polypeptide 3 ( TCEB3 ), and peroxisome proliferative activated receptor delta ( PPARD ) were identified. Of the more than twofold downregulated genes, PHD finger protein 15 ( PHF15 ) and zinc finger protein 33a ( ZNF33A ) were identified. In summary, identification of these genes by cDNA microarray analysis in response to melatonin administration may provide a foundation for further studies on the function of melatonin in human PBMCs.