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Expression of N ‐methyl‐ D ‐aspartate (NMDA) and α ‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionate (AMPA) GluR2/3 receptors in the developing rat pineal gland
Author(s) -
Kaur C.,
Sivakumar V.,
Ling E. A.
Publication year - 2005
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/j.1600-079x.2005.00245.x
Subject(s) - ampa receptor , pineal gland , receptor , medicine , glial fibrillary acidic protein , biology , nmda receptor , endocrinology , glutamate receptor , immunofluorescence , melatonin , immunohistochemistry , astrocyte , microglia , neuroglia , microbiology and biotechnology , immunology , central nervous system , biochemistry , inflammation , antibody
The expression of α ‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionate (AMPA) type glutamate (GluR2/3) receptors and N ‐methyl‐ d ‐aspartate receptor subtype 1 (NMDAR1) was carried out by immunohistochemistry, double immunofluorescence and real‐time RT‐PCR analysis in the pineal glands of 1‐day to 6‐wk‐old rats in the present study. GluR2/3 immunopositive cells were distributed throughout the pineal gland and showed branching processes in all age groups. The NMDAR1 immunoreactivity, however, was observed in fewer branched cells. A constitutive mRNA expression of NMDAR1, GluR2 and GluR3 was detected in the pineal glands of various ages and showed no significant difference between the age groups studied. Immunohistochemical and double immunofluorescence results showed that the GluR2/3 were mainly expressed and co‐localized with OX‐42‐positive microglia/macrophages and the glial fibrillary acidic protein (GFAP)‐positive astrocytes. Co‐localization of NMDAR1 with OX‐42‐ and GFAP‐positive cells was much less. The expression of these receptors on the glial cells suggests that they may be involved in the development and growth of the pineal gland in the early postnatal period (1 day to 3 wk) and subsequently in the regulation of melatonin synthesis.