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Dual effect of melatonin as proinflammatory and antioxidant in collagen‐induced arthritis in rats
Author(s) -
JiménezCaliani Antonio J.,
JiménezJorge Silvia,
Molinero Patrocinio,
Guerrero Juan M.,
FernándezSantos Jose M.,
MartínLacave Inés,
Osuna Carmen
Publication year - 2005
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/j.1600-079x.2004.00175.x
Subject(s) - melatonin , proinflammatory cytokine , endocrinology , medicine , chemistry , lipid peroxidation , antioxidant , nitrite , arthritis , inflammation , nitrate , biochemistry , organic chemistry
The aim of this study was to determine the effects of melatonin on proinflammatory status of rats with collagen‐induced arthritis (CIA). CIA was induced in male Wistar rats with an emulsion of type II collagen in Freund's Incomplete Adjuvant (C‐II/FIA). For 14 days, control and pinealectomized rats received a subcutaneous injection of 100 μ L melatonin (30 μ g) or vehicle (saline on 1% ethanol). Levels of cytokines interleukin (IL)‐1 β and IL‐6 were determined in the serum, peripheral blood mononuclear cells, and joints. Levels of anti‐type II collagen antibody, nitrite/nitrate, and lipid peroxidation (LPO) were determined in the serum, joints, and brain. Treatment with melatonin significantly increased the levels of IL‐1 β , IL‐6, nitrite/nitrate and LPO in joints. However, melatonin significantly reduced the levels of nitrite/nitrate and LPO in serum and brain. Moreover, CIA in pinealectomized rats presented significantly reduced levels of IL‐1 β and IL‐6, titers of anti‐type II collagen antibodies, levels of nitrite/nitrate, and LPO in joints but elevated levels in serum and brain. Melatonin has been described as a proinflammatory and antioxidant agent. In a process of inflammation as CIA, melatonin acts with a markedly proinflammatory effect at local and peripheral levels maintaining its antioxidant effect only at peripheral level.