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Expression of the melatonin receptor in Xenopus laevis : A comparative study between protein and mRNA distribution
Author(s) -
Mazzucchelli Cristina,
Capsoni Simona,
Angeloni Deborah,
Fraschini Franco,
Stankov Bojidar
Publication year - 1996
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/j.1600-079x.1996.tb00240.x
Subject(s) - xenopus , biology , melatonin receptor , melatonin , messenger rna , in situ hybridization , microbiology and biotechnology , gene expression , receptor , rna , pineal gland , gene , endocrinology , biochemistry
Reverse transcription‐polymerase chain reaction (RT‐PCR) analysis performed on total RNA from different tissues of Xenopus laevis showed that the melatonin receptor gene cloned from dermal melanophores is expressed in the whole brain, skin, and retina, and that apart from the ovary, there is no expression in tissues having origin outside the central nervous system. Comparative studies using in vitro autoradiography and in situ hybridization demonstrated that the melatonin receptor is expressed with discrete allocation in Xenopus brain. Though the distribution pattern of the specific messenger RNA conforms well with that of the corresponding receptor protein, it is not always coincident.