Premium
Localization and characterization of [ 125 I]iodomelatonin binding sites in duck gonads
Author(s) -
Ayre Elizabeth A.,
Wang Zheng Pin,
Brown Gregory M.,
Pang Shiu Fun
Publication year - 1994
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/j.1600-079x.1994.tb00112.x
Subject(s) - radioligand , melatonin , binding site , endocrinology , medicine , biology , dissociation constant , radioligand assay , biochemistry , receptor
Ayre EA, Wang ZP, Brown GM, Pang SF. Localization and characterization of [ 125 I]iodomelatonin binding sites in duck gonads. J. Pineal Res. 1994: 17: 39–47. Abstract The characterization and localization of [ 125 I]iodomelatonin binding sites in the gonads advances the understanding of possible regulatory sites of melatonin action. With the availability of [ 125 I]iodomelatonin as a biologically active radioligand, our study utilized a combined approach of autoradiography for anatomical resolution together with an established radioligand binding assay to assess mid‐light [ 125 I]iodomelatonin binding in the testes and ovaries of ducks. In the autoradiography study, specific [ 125 I]iodomelatonin binding was shown to be homogeneous throughout the testes, while in the ovaries, specific [ 125 I]iodomelatonin binding appeared to be concentrated around the follicle. Radioligand binding assay results indicated a single class of binding sites with the maximum number of [ 125 I]iodomelatonin binding sites measured at 1. 91 ± 0. 70 fmol/mg protein in testicular membrane and 4. 54 ± 0. 64 fmol/mg protein in ovarian membrane. [ 125 I]Iodomelatonin binding affinity, characterized by equilibrium dissociation constants of 29 ± 6 pmol/L in testicular membrane and 53 ± 9 pmol/L in ovarian membrane, was in accordance with circulating melatonin levels, suggesting an appropriate concentration for eliciting a physiological response. [I]Iodomelatonin binding in duck gonads satisfied all the criteria for a binding site, being rapid, stable, saturable, reversible, specific, and of high affinity, and may indicate a direct pineal‐gonadal connection.