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Expression of somatostatin in rat pineal cells in culture
Author(s) -
Mato E.,
Santisteban P.,
Viader M.,
Capellá G.,
Fornas O.,
PuigDomingo M.,
Webb S.M.
Publication year - 1993
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/j.1600-079x.1993.tb00508.x
Subject(s) - somatostatin , pineal gland , reverse transcription polymerase chain reaction , messenger rna , melatonin , biology , complementary dna , medicine , neuropeptide , endocrinology , somatostatin receptor 3 , cell culture , somatostatin receptor 2 , microbiology and biotechnology , chemistry , somatostatin receptor , biochemistry , gene , receptor , genetics
The expression of somatostatin mRNA was investigated in rat pineal cells after 1 week in culture, using reverse transcription of mRNA into cDNA and the polymerase chain reaction. The positive expression in cultured pineal cells demonstrates the capacity of this gland to synthesize somatostatin in denervated cells. Thus, apart from the neural origin of pineal somatostatin, which has been described in detail in the bovine species, a parenchymal source is demonstrated.