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Antiproliferative effect of pineal indoles on cultured tumor cell lines
Author(s) -
Sze S.F.,
Ng T.B.,
Liu W.K.
Publication year - 1993
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/j.1600-079x.1993.tb00481.x
Subject(s) - melatonin , pineal gland , cell culture , cell growth , biology , chemistry , medicine , microbiology and biotechnology , endocrinology , biochemistry , genetics
The in vitro antiproliferative action of pineal indoles on several tumor cell lines including melanoma (B16), sarcoma (S180), macrophage‐like cell line (PU5), fibroblasts (3T3), and choriocarcinoma (JAr) was examined by measuring the incorporation of 3 H‐thymidine by the tumor cells, and, in the case of melanoma cells, by also measuring the incorporation of 3 H‐leucine and 3 H‐uridine. Uptake of crystal violet was used to assess the viability of the tumor cells. The order of inhibitory potency of the indoles was found to be methoxytryptamine > melatonin, methoxytryptophol, hydroxytryptophol, and methoxyindoleacetic acid > serotonin and hydroxyindoleactic acid. The possibility of an adverse effect of the indoles on the viability of normal cells was also investigated by employing a primary culture of rat hepatocytes. The release of glutamate‐oxaloacetate transaminase by hepatocytes was not affected by the indoles, although the release of glutamate‐pyruvate transaminase was increased to a small extent and the uptake of crystal violet was slightly inhibited