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Purinergic receptors have no major role in control of the circadian rhythm in rate of thymidine incorporation by cultured chick pineal glands
Author(s) -
Wainwright S.D.,
Wainwright Lillian K.
Publication year - 1991
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/j.1600-079x.1991.tb00814.x
Subject(s) - adenosine , thymidine , medicine , endocrinology , biology , purinergic receptor , pineal gland , adenosine receptor , circadian rhythm , adenosine deaminase , deoxyadenosine , adenosine receptor antagonist , receptor , biochemistry , agonist , in vitro
We have examined the effects of some analogues of adenosine upon the circadian rhythm in rate of thymidine incorporation by cultured chick pineal glands. Incorporation in the early period of the photoperiod on day 2 of culture was slightly inhibited by the adenosine analogue N‐ethylcarboxamido‐adenosine, but this effect was not countered by the antagonist 8‐phenyl‐theophylline. Thymidine incorporation was inhibited when glands were continuously exposed to the adenosine transport inhibitor nitrobenzyl‐thioinosine, but ongoing incorporation was not inhibited by addition of this agent. Removal of adenosine and deoxyadenosine supplements from the medium, with or without further addition of adenosine deaminase, had no appreciable effects upon thymidine incorporation. We conclude that adenosine and analogues probably play no role in regulation of the rhythm in rate of thymidine incorporation.