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Thymidine Incorporation by Chick Pineal Glands as Studied by Pulse‐Labelling In Vitro
Author(s) -
Wainwright S. D.,
Wainwright Lillian K.
Publication year - 1990
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/j.1600-079x.1990.tb00696.x
Subject(s) - labelling , thymidine , biology , in vitro , pulse (music) , circadian rhythm , medicine , endocrinology , biochemistry , optics , physics , detector
We have studied the pattern of variations in extent of thymidine incorporation during pulse‐labelling in cultured chick pineal glands. During the first 24‐30 h in culture the extent of pulse‐labelling varied in parallel with the cycle in cumulative incorporation. Pulse‐labelling was seen during the period of apparent arrest of cumulative incorporation. However, it was probably “masked” by the SD of assays of cumulative incorporation and represented a minor, but distinct, process of thymidine incorporation. The pattern of variation in extent of pulse‐labelling was qualitatively consistent under a wide variety of conditions. Control of this pattern appeared to be different from that of the circadian rhythm in cumulative thymidine incorporation.