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Histoenzymological Light and Electron Microscopic Localization of Carbonic Anhydrase in the Rat Superficial Pineal Gland
Author(s) -
Krstic Radivoj,
Nicolas Dominique
Publication year - 1990
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/j.1600-079x.1990.tb00672.x
Subject(s) - carbonic anhydrase , pineal gland , melatonin , electron microscope , biology , carbonic anhydrase ii , chemistry , anatomy , enzyme , biophysics , biochemistry , physics , endocrinology , optics
A histoenzymological method for demonstration of carbonic anhydrase (CA) was used for cryostat sections of superficial rat pineal gland. Light and electron microscopic analysis showed a high concentration of this enzyme in stellate cells, particularly heavily distributed under the organ capsule and scattered in the parenchyma, where they form a widespread three‐dimensional network. Most of the pericapillary spaces were lined by CA‐reactive cells. The reaction product, in the form of strongly electron‐dark precipitates, was localized in the cytoplasmic matrix, whereas organelles, except for some mitochondrial intracristal spaces, were CA‐negative. On the basis of localization and morphology of CA‐positive cells, we conclude that these represent glial (interstitial) cells.