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Partial Purification of a Polypeptide Extract Derived From Ovine Pineal That Suppresses the Growth of Human Melanoma Cells In Vitro
Author(s) -
Noteborn H.P.J.M.,
Weusten J.J.A.M.,
Bartsch H.,
Bartsch C.,
Flehmig B.,
Ebels I.,
Salemink C.A.
Publication year - 1989
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/j.1600-079x.1989.tb00435.x
Subject(s) - melatonin , in vitro , pineal gland , size exclusion chromatography , melanoma , cell culture , biology , biological activity , chemistry , endocrinology , biochemistry , medicine , cancer research , genetics , enzyme
A combination of gel filtration and reverse‐phase high performance liquid chromatography with postcolumn antitumour assay has been developed. A melatonin insensitive human melanoma cell strain was used to guide the purification of the antitumour effect of an ovine pineal aqueous extract (MW 1,000 to 10,000) that possessed the ability to decrease the hypophysiotropic activity of rat and mice hypothalami in vitro. This allows a specific identification of a pined factor (MW 2,000 to 6,000) that inhibits the growth of human melanoma cells at a dose of 0.47 mg/ml medium. It was shown that the activity of this pineal compound differs from structures known to be present in the pineal, such as melatonin, pteridines, and β‐carbolines. There appears to be evidence for a peptidic nature of this pineal antitumour factor.