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Topical nutlin‐3a does not decrease photocarcinogenesis induced by simulated solar radiation in hairless mice
Author(s) -
Lerche Catharina Margrethe,
Philipsen Peter Alshede,
Poulsen Thomas,
Gniadecki Robert,
Wulf Hans Christian
Publication year - 2012
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/j.1600-0781.2012.00675.x
Subject(s) - hairless , erythema , cancer research , skin cancer , medicine , dermatology , dna damage , pharmacology , cancer , chemistry , dna , biochemistry
Summary Background Nutlin‐ 3a increases p53 levels after UVB radiation, which could result in a decrease in DNA damage and thus lead to a lower risk of non‐melanoma skin cancer. Especially, organ transplant recipients might derive benefit from such a topical formulation with an active ingredient to prevent DNA damage. Purpose To investigate whether topical nutlin‐ 3a can decrease photocarcinogenesis induced by simulated solar radiation. Methods 72 hairless C3.Cg/TifBomTac mice were treated 3 days/week topically with 100 μl nutlin‐ 3a (9 mM ) [ G roups 1 and 3 (120 days)) or 100 μl vehicle ( G roup 2). Three hours later, all mice were exposed to simulated solar radiation (a radiometric equivalent of three standard erythema dose units). Results The median time to tumours did not differ between the mice treated with nutlin‐ 3a and with the vehicle. The median time to the first and second tumours did not differ between ‘nutlin‐ 3a ‐120 days’ and vehicle‐treated mice, but there was a small significant difference in the median time to the third tumour (211 vs. 196 days, P = 0.043). However, after B onferroni correction, there was no difference at all. Conclusion Nutlin‐ 3a had no reductive effect on photocarcinogenesis and we do not believe in nutlin‐ 3a as a potential drug against DNA damage in a topical formulation for organ transplant patients.