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Effect of hematoporphyrin monomethyl ether‐mediated photodynamic therapy on hypertrophic scar fibroblasts
Author(s) -
Cai Hong,
Gu Ying,
Sun Qiang,
Zeng Jing,
Dong Ning,
Zhao Guang
Publication year - 2011
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/j.1600-0781.2011.00577.x
Subject(s) - photodynamic therapy , hematoporphyrin , apoptosis , photosensitizer , hypertrophic scar , chemistry , flow cytometry , fibroblast , staining , sonodynamic therapy , annexin , microbiology and biotechnology , caspase 3 , immunofluorescence , cancer research , pathology , in vitro , medicine , biology , immunology , programmed cell death , biochemistry , antibody , organic chemistry
Background: Clinical studies have demonstrated that photodynamic therapy (PDT) for hyperplastic dermatosis results in a beneficial outcome. Hypertrophic scar (HS) is a pathological process characterized by fibroblastic hyperproliferation. However, it is unclear whether photochemical interactions between PDT and fibroblasts contribute to a beneficial outcome. To investigate the primary photochemical effects of PDT, we studied the efficacy of 630 nm PDT on human fibroblasts from HS using hematoporphyrin monomethyl ether (HMME) as a photosensitizer. Methods: Fibroblasts were cultured from nontreated HSs, and cells at passage 4–6 were used for the experiments. Morphological and biochemical changes in fibroblasts were assessed by Hoechst 33258 staining and annexin V‐FITC/PI flow cytometry (FCM). Caspase‐3 activity assay and immunofluorescence staining were performed to investigate caspase‐3 expression in fibroblasts. Results: The morphological features of cell apoptosis were viewed under a fluorescent microscope by Hoechst 33258 staining. FCM indicated that the apoptotic rate was significantly increased after HMME–PDT, and caspase‐3 activity was observed. Conclusions: Low‐level exposure to 630 nm PDT mediated by HMME appears to induce fibroblast apoptosis and stimulate caspase‐3 activation. However, the effect of HMME–PDT on fibroblasts needs further investigation to determine its therapeutic potential for HS.