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Mitigation of acute ultraviolet B radiation‐mediated damages by baicalin in mouse skin
Author(s) -
Zhou BingRong,
Lin BingJiang,
Jin SongLiang,
Luo Dan
Publication year - 2009
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/j.1600-0781.2009.00454.x
Subject(s) - baicalin , hairless , sunburn , pyrimidine dimer , skin cancer , dna damage , chemistry , human skin , dermatology , photoaging , in vivo , erythema , pharmacology , medicine , biochemistry , biology , cancer , dna , genetics , high performance liquid chromatography , microbiology and biotechnology , chromatography
Background: Solar ultraviolet (UV) irradiation, in particular UVB with a wavelength range between 290 and 320 nm, induces different hazardous effects on the skin, including sunburn, photoaging and cancer. Protection against sun‐induced damage is therefore a highly desirable goal. Chemoprevention is being investigated as a potential approach for the management of UV damages including skin cancer. Aim: In this study, to determine the relevance of our in vitro findings to in vivo situations, we assessed the effects of baicalin on UVB‐mediated damages in mice skin. Methods: Balb/C hairless mice were topically pretreated (24 h before UVB) or post‐treated (5 min after UVB) with baicalin (1 mg/cm 2 skin area/mouse/100 μl acetone) and were exposed to UVB 24 h later (180 mJ/cm 2 ). The animals were sacrificed 1 and 24 h after the UVB exposure. Skin edema, histopathology changes, hydrogen peroxide (H 2 O 2 ) and cyclobutane pyrimidine dimers (CPDs)‐positive cells were assessed to determine the UVB‐induced photodamage. Results: Our data demonstrated that a topical application of baicalin, either as a pretreatment or as a post‐treatment, resulted in a significant decrease in UVB mediated increases in skin edema, skin hyperplasia and infiltration of leukocytes. Further, baicalin treatments (pre and post) also resulted in a significant decrease in UVB mediated (1) generation of H 2 O 2 and (2) formation of DNA photolesions: CPDs. Conclusion: Based on these data, we suggest that baicalin could be developed as an agent for the management of conditions elicited by UV exposure including skin cancer.