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Protective effect of the Baicalin against DNA damage induced by ultraviolet B irradiation to mouse epidermis
Author(s) -
BingRong Zhou,
SongLiang Jin,
XiaoE Chen,
XiangFei Lin,
BaoXiang Cai,
Jie Gao,
Dan Luo
Publication year - 2008
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/j.1600-0781.2008.00356.x
Subject(s) - baicalin , tunel assay , dna damage , epidermis (zoology) , pyrimidine dimer , apoptosis , microbiology and biotechnology , blot , terminal deoxynucleotidyl transferase , chemistry , immunohistochemistry , photolyase , dna , biology , dna repair , biochemistry , immunology , gene , anatomy , high performance liquid chromatography , chromatography
Purpose: To investigate whether topical application of Baicalin affords protecting Balb/C mice epidermis from ultraviolet (UV)B‐induced DNA damage and its underlying mechanisms. Methods: A DNA damage model of UVB irradiation‐induced mice epidermis was established. The immunohistochemical staining, Southwestern dot‐blotting were used for cyclobutane pyrimidine dimers (CPDs) detection; Western blotting was used for p53 detection; reverse transcription‐polymerase chain reaction (RT‐PCR) was used to detect the mRNA level of Bcl‐2 and Bax; terminal deoxynucleotidyl transferase‐mediated dUTP nick end labeling (TUNEL) was used to detect apoptotic cells. Results: Topical application of Baicalin on Balb/C mice skin significantly decreased the amount of epidermal CPDs 1, 24 and 48 h after 180 mJ/cm 2 of UVB irradiation as compared with untreated mice. UVB‐induced apoptosis was less pronounced in Baicalin‐treated mice epidermis, which was accompanied by less p53 accumulation and higher Bcl‐2/Bax ratio compared with that of untreated mice. Conclusion: Taken together, these results suggest that topical Baicalin application mitigates DNA photo‐damage. Baicalin is therefore a promising protective substance against UVB radiation.