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Thioredoxin upregulation by 5‐aminolaevulinic acid‐based photodynamic therapy in human skin squamous cell carcinoma cell line
Author(s) -
Kuhara Tomoe,
Watanabe Daisuke,
Akita Yoichi,
Takeo Tomohiro,
Ishida Natsuko,
Nakano Aki,
Yamashita Noriko,
Ohshima Yuichiro,
Kawada Morihiro,
Yanagishita Takeshi,
Tamada Yasuhiko,
Matsumoto Yoshinari
Publication year - 2008
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/j.1600-0781.2008.00354.x
Subject(s) - thioredoxin , apoptosis , photodynamic therapy , programmed cell death , cancer research , annexin , cell growth , cell culture , microbiology and biotechnology , propidium iodide , biology , chemistry , pathology , medicine , oxidative stress , biochemistry , genetics , organic chemistry
Summary Background/purpose: 5‐aminolaevulinic acid‐based photodynamic therapy (ALA‐PDT) is widely performed in the clinical setting for superficial skin cancers, giving favorable results, but residual tumor and recurrence occur occasionally. Thioredoxin is a common antioxidant that suppresses apoptosis and facilitates cell growth. We investigated the expression of thioredoxin following ALA‐PDT in human skin squamous cell carcinoma cell line, HSC‐5. Methods: ALA‐PDT was performed in HSC‐5 cells using low‐dose (5 J/cm 2 , 100 mW/cm 2 ) or high‐dose (30 J/cm 2 , 100 mW/cm 2 ) irradiation, and the expression of thioredoxin was measured by Western blotting. An MTT assay was used to assess cell growth following a low dose of multiple irradiations. Cell death was examined by Western blotting for caspase‐3 and PARP. Immunofluorescence double staining using annexin V and propidium iodine was also performed. Results: Expression of thioredoxin was only observed following low‐dose exposure ALA‐PDT. Multiple low‐dose exposure ALA‐PDT significantly proliferated cell growth. With high‐dose exposure ALA‐PDT, caspase‐3 and PARP expression were seen, and cell death due to apoptosis and/or necrosis was observed, but thioredoxin was barely detected. Conclusion: Low‐dose exposure ALA‐PDT increased the expression of thioredoxin and facilitated the growth of HSC‐5 cells.