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Ultraviolet A exposure alters adhesive properties of mouse melanoma cells
Author(s) -
Pastila Riikka,
Leszczynski Dariusz
Publication year - 2005
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/j.1600-0781.2005.00166.x
Subject(s) - melanoma , irradiation , cell , in vitro , cell culture , chemistry , microbiology and biotechnology , adhesion , cell adhesion , cancer research , medicine , biology , biochemistry , physics , organic chemistry , nuclear physics , genetics
Background: We have examined whether ultraviolet A (UVA) irradiation could alter adhesive properties of melanoma cells. As an experimental in vitro model, we have used C57BL/6 mouse‐derived B16‐ F1 and B16‐F10 melanoma cell lines and the syngeneic MS‐1 endothelial cell line. Method/Result: The melanoma cells were exposed to different doses of UVA irradiation. We have determined that a single dose of UVA at 8 and 12 J/cm 2 causes an 88% ( P <0.001) and a 32% ( P <0.05) increase in B16‐F1 melanoma cell adhesiveness to the non‐irradiated endothelial monolayer, respectively. The peak of the response was 24 h after the irradiation. The UVA dose of 8 J/cm 2 delivered in four doses separated by 1 h intervals (4 × 2 J/cm 2 ) had led to a caused 149% ( P <0.001) increase of B16‐F1 melanoma adhesiveness already at 1 h after the last dose of UVA. Besides the induction of increase in the melanoma–endothelial cell adhesion, UVA exposure has induced a rapid decline (1 h after exposure) in homotypic melanoma–melanoma cell adhesion (clustering). The clustering decline of B16‐F1 cells with a single dose of UVA at 8 J/cm 2 was by 61% ( P <0.05) and by 35% ( P <0.05) with 4 × 2 J/cm 2 . Pilot experiments have shown that the changes of the adhesive properties of melanoma cells were accompanied by an increase in N‐cadherin expression and a decline in E‐cadherin expression. Such a change in cadherin expression profile has been shown to be an indicator of the increased metastatic potential. Conclusion: Our results suggest that UVA radiation appears to alter the adhesive properties of melanoma cells in vitro , by diminishing the melanoma–melanoma adhesion and by increasing melanoma adhesion to the endothelium. This suggests that UVA exposure might increase the metastatic capability of the melanoma cells.