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A study of Q‐switched Nd:YAG laser irradiation and paracrine function in human skin cells
Author(s) -
Burd Andrew,
Zhu Ningwen,
Poon Vincent K. M.
Publication year - 2005
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/j.1600-0781.2005.00155.x
Subject(s) - paracrine signalling , hepatocyte growth factor , downregulation and upregulation , fibroblast growth factor , fibroblast , basic fibroblast growth factor , cell culture , microbiology and biotechnology , stem cell factor , autocrine signalling , cytokine , keratinocyte , biology , cancer research , growth factor , chemistry , receptor , immunology , stem cell , progenitor cell , biochemistry , gene , genetics
Background and objectives: This preliminary laboratory‐based study looks at the paracrine release from human skin cells subject to sublethal Q‐switched Nd:YAG 532 nm laser irradiation. Study design/Materials and methods: Human dermal fibroblast and keratinocyte cultures were exposed to sublethal energy using the Nd:YAG 532 nm laser. Altered gene expression was then screened using RT‐PCR for a range of paracrine factors known to affect melanogenesis, basic fibroblast growth factor (b‐FGF), hepatocyte growth factor (HGF), stem cell factor (SCF), melanocyte stimulating hormone (MSH), endothelin‐1 (ET‐1), interleukin‐6 (IL‐6), tumor necrosis factor‐α (TNF‐α) and protease‐activated receptor‐2 (PAR‐2). Enzyme‐linked immunosorbent assay (ELISA) was used to confirm protein production. Conditioned medium was used to assess altered melanogenesis in a melanoma cell line. Results: Fibroblasts exposed to sublethal radiation showed upregulation of b‐FGF, HGF and SCF. This contrasts with keratinocytes which showed upregulation of IL‐6. Elevated protein levels of b‐FGF and SCF were confirmed by ELISA assay. Conditioned fibroblast medium was shown to stimulate melanogenesis in a melanoma cell line. Conclusions: This preliminary laboratory study reports, for the first time, specific gene upregulation using the Q‐switched Nd:YAG 532 nm laser.